Transcriptomic analyses reveal the expression and regulation of genes associated with resistance to early leaf spot in peanut.
Identifieur interne : 000013 ( Main/Exploration ); précédent : 000012; suivant : 000014Transcriptomic analyses reveal the expression and regulation of genes associated with resistance to early leaf spot in peanut.
Auteurs : Limin Gong [États-Unis] ; Suoyi Han [République populaire de Chine] ; Mei Yuan [République populaire de Chine] ; Xingli Ma [République populaire de Chine] ; Austin Hagan [États-Unis] ; Guohao He [États-Unis]Source :
- BMC research notes [ 1756-0500 ] ; 2020.
Abstract
OBJECTIVE
Early leaf spot (ELS) caused by Cercospora arachidicola (Hori) is a serious foliar disease in peanut worldwide, which causes considerable reduction of yield. Identification of resistance genes is important for both conventional and molecular breeding. Few resistance genes have been identified and the mechanism of defense responses to this pathogen remains unknown.
RESULTS
We detected several genes involved in disease resistance to ELS through transcriptome analysis. Using RNA-seq technology, one hundred thirty-three differentially expressed genes (DEGs) were identified between resistant and susceptible lines. Among these DEGs, coiled coil-nucleotide binding-leucine rich repeat (NLR) type resistance genes were identified as duplicated R genes on the chromosome B2. Peanut phytoalexin deficient 4 (PAD4) regulator of effector-triggered immunity mediated by NLR resistance proteins and polyphenol oxidase (PPO) genes play important roles in early leaf spot resistance. Our study provides the useful information on plant response to C. arachidicola infection in peanut. The results suggest that a few major genes and several factors mediate the resistance to ELS disease, showing the characteristics of quantitative trait in defense responses.
DOI: 10.1186/s13104-020-05225-9
PubMed: 32782019
PubMed Central: PMC7418390
Affiliations:
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Austin" sort="Hagan, Austin" uniqKey="Hagan A" first="Austin" last="Hagan">Austin Hagan</name><affiliation wicri:level="1"><nlm:affiliation>Auburn University, Auburn, AL, 36830, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Auburn University, Auburn, AL, 36830</wicri:regionArea><wicri:noRegion>36830</wicri:noRegion></affiliation></author><author><name sortKey="He, Guohao" sort="He, Guohao" uniqKey="He G" first="Guohao" last="He">Guohao He</name><affiliation wicri:level="1"><nlm:affiliation>Tuskegee University, Tuskegee, AL, 36088, USA. ghe@tuskegee.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Tuskegee University, Tuskegee, AL, 36088</wicri:regionArea><wicri:noRegion>36088</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:32782019</idno><idno type="pmid">32782019</idno><idno 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36088</wicri:regionArea><wicri:noRegion>36088</wicri:noRegion></affiliation></author><author><name sortKey="Han, Suoyi" sort="Han, Suoyi" uniqKey="Han S" first="Suoyi" last="Han">Suoyi Han</name><affiliation wicri:level="1"><nlm:affiliation>Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Henan Academy of Agricultural Sciences, Zhengzhou, 450002</wicri:regionArea><wicri:noRegion>450002</wicri:noRegion></affiliation></author><author><name sortKey="Yuan, Mei" sort="Yuan, Mei" uniqKey="Yuan M" first="Mei" last="Yuan">Mei Yuan</name><affiliation wicri:level="1"><nlm:affiliation>Shandong Peanut Research Institute, Qingdao, 266000, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Shandong Peanut Research Institute, Qingdao, 266000</wicri:regionArea><wicri:noRegion>266000</wicri:noRegion></affiliation></author><author><name sortKey="Ma, Xingli" sort="Ma, Xingli" uniqKey="Ma X" first="Xingli" last="Ma">Xingli Ma</name><affiliation wicri:level="1"><nlm:affiliation>Henan Agricultural University, Zhengzhou, 450002, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Henan Agricultural University, Zhengzhou, 450002</wicri:regionArea><wicri:noRegion>450002</wicri:noRegion></affiliation></author><author><name sortKey="Hagan, Austin" sort="Hagan, Austin" uniqKey="Hagan A" first="Austin" last="Hagan">Austin Hagan</name><affiliation wicri:level="1"><nlm:affiliation>Auburn University, Auburn, AL, 36830, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Auburn University, Auburn, AL, 36830</wicri:regionArea><wicri:noRegion>36830</wicri:noRegion></affiliation></author><author><name sortKey="He, Guohao" sort="He, Guohao" uniqKey="He G" first="Guohao" last="He">Guohao He</name><affiliation wicri:level="1"><nlm:affiliation>Tuskegee University, Tuskegee, AL, 36088, USA. ghe@tuskegee.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Tuskegee University, Tuskegee, AL, 36088</wicri:regionArea><wicri:noRegion>36088</wicri:noRegion></affiliation></author></analytic><series><title level="j">BMC research notes</title><idno type="eISSN">1756-0500</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>OBJECTIVE</b></p><p>Early leaf spot (ELS) caused by Cercospora arachidicola (Hori) is a serious foliar disease in peanut worldwide, which causes considerable reduction of yield. Identification of resistance genes is important for both conventional and molecular breeding. Few resistance genes have been identified and the mechanism of defense responses to this pathogen remains unknown.</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>We detected several genes involved in disease resistance to ELS through transcriptome analysis. Using RNA-seq technology, one hundred thirty-three differentially expressed genes (DEGs) were identified between resistant and susceptible lines. Among these DEGs, coiled coil-nucleotide binding-leucine rich repeat (NLR) type resistance genes were identified as duplicated R genes on the chromosome B2. Peanut phytoalexin deficient 4 (PAD4) regulator of effector-triggered immunity mediated by NLR resistance proteins and polyphenol oxidase (PPO) genes play important roles in early leaf spot resistance. Our study provides the useful information on plant response to C. arachidicola infection in peanut. The results suggest that a few major genes and several factors mediate the resistance to ELS disease, showing the characteristics of quantitative trait in defense responses.</p></div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">32782019</PMID><DateRevised><Year>2020</Year><Month>08</Month><Day>14</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1756-0500</ISSN><JournalIssue CitedMedium="Internet"><Volume>13</Volume><Issue>1</Issue><PubDate><Year>2020</Year><Month>Aug</Month><Day>11</Day></PubDate></JournalIssue><Title>BMC research notes</Title><ISOAbbreviation>BMC Res Notes</ISOAbbreviation></Journal><ArticleTitle>Transcriptomic analyses reveal the expression and regulation of genes associated with resistance to early leaf spot in peanut.</ArticleTitle><Pagination><MedlinePgn>381</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1186/s13104-020-05225-9</ELocationID><Abstract><AbstractText Label="OBJECTIVE" NlmCategory="OBJECTIVE">Early leaf spot (ELS) caused by Cercospora arachidicola (Hori) is a serious foliar disease in peanut worldwide, which causes considerable reduction of yield. Identification of resistance genes is important for both conventional and molecular breeding. Few resistance genes have been identified and the mechanism of defense responses to this pathogen remains unknown.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">We detected several genes involved in disease resistance to ELS through transcriptome analysis. Using RNA-seq technology, one hundred thirty-three differentially expressed genes (DEGs) were identified between resistant and susceptible lines. Among these DEGs, coiled coil-nucleotide binding-leucine rich repeat (NLR) type resistance genes were identified as duplicated R genes on the chromosome B2. Peanut phytoalexin deficient 4 (PAD4) regulator of effector-triggered immunity mediated by NLR resistance proteins and polyphenol oxidase (PPO) genes play important roles in early leaf spot resistance. Our study provides the useful information on plant response to C. arachidicola infection in peanut. The results suggest that a few major genes and several factors mediate the resistance to ELS disease, showing the characteristics of quantitative trait in defense responses.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Gong</LastName><ForeName>Limin</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Tuskegee University, Tuskegee, AL, 36088, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Han</LastName><ForeName>Suoyi</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yuan</LastName><ForeName>Mei</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Shandong Peanut Research Institute, Qingdao, 266000, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ma</LastName><ForeName>Xingli</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Henan Agricultural University, Zhengzhou, 450002, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hagan</LastName><ForeName>Austin</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Auburn University, Auburn, AL, 36830, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>He</LastName><ForeName>Guohao</ForeName><Initials>G</Initials><Identifier Source="ORCID">http://orcid.org/0000-0001-5350-7786</Identifier><AffiliationInfo><Affiliation>Tuskegee University, Tuskegee, AL, 36088, USA. ghe@tuskegee.edu.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>2014-38821-22453</GrantID><Agency>USDA-NIFA</Agency><Country></Country></Grant><Grant><GrantID>31871663</GrantID><Agency>National Natural Science Foundation of China</Agency><Country></Country></Grant><Grant><GrantID>182102110137</GrantID><Agency>Major R&D and Promotion Projects in Henan, China</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>08</Month><Day>11</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>BMC Res Notes</MedlineTA><NlmUniqueID>101462768</NlmUniqueID><ISSNLinking>1756-0500</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Defense response</Keyword><Keyword MajorTopicYN="N">Differentially expressed gene</Keyword><Keyword MajorTopicYN="N">Gene regulation</Keyword><Keyword MajorTopicYN="N">RNA-seq</Keyword><Keyword MajorTopicYN="N">Transcriptome</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate 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Chine</li><li>États-Unis</li></country></list><tree><country name="États-Unis"><noRegion><name sortKey="Gong, Limin" sort="Gong, Limin" uniqKey="Gong L" first="Limin" last="Gong">Limin Gong</name></noRegion><name sortKey="Hagan, Austin" sort="Hagan, Austin" uniqKey="Hagan A" first="Austin" last="Hagan">Austin Hagan</name><name sortKey="He, Guohao" sort="He, Guohao" uniqKey="He G" first="Guohao" last="He">Guohao He</name></country><country name="République populaire de Chine"><noRegion><name sortKey="Han, Suoyi" sort="Han, Suoyi" uniqKey="Han S" first="Suoyi" last="Han">Suoyi Han</name></noRegion><name sortKey="Ma, Xingli" sort="Ma, Xingli" uniqKey="Ma X" first="Xingli" last="Ma">Xingli Ma</name><name sortKey="Yuan, Mei" sort="Yuan, Mei" uniqKey="Yuan M" first="Mei" last="Yuan">Mei Yuan</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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